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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been observed in many tumors, which leads to chromosome instability, and so may perhaps lead to tumorigenesis. This protein was located to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a crucial role in endorsing cell-cycle development during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, happen to be explained. Two further splice variants had been claimed but in depth nucleotide sequence info isn't nonetheless accessible.
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Blocking peptides are peptides that bind specially into the focus on antibody and block antibody binding. These peptide typically consists of the epitope regarded via the antibody.
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This guidebook outlines further attainable optimization of mobile and immunoassay parameters to be certain the very best outcomes are...
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This information outlines more probable optimization of cellular and immunoassay parameters to make certain the very best effects are...
Antibodies bound to the blocking peptide not bind to the epitope about the concentrate on protein. By comparing website the staining with the blocked antibody versus the antibody on your own, you can see which staining is unique.
Focus on Info Cyclin E belongs towards the highly conserved cyclin family members, whose members are characterized by a extraordinary periodicity in protein abundance throughout the mobile cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show unique expression and degradation styles which contribute towards the temporal coordination of each and every mitotic function. Cyclin E types a posh with and functions as a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S transition. This protein accumulates in the G1-S period boundary and is also degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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